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Alkaline Phosphatase Isoenzymes High Resolution Proteins Immunofixation Immunoelectrophoresis The alkaline phosphatase ALP isoenzymes found in human serum originate from several kpotreba with the greatest ge, occurring in the bone, liver, intestine, and placenta. Because of wide distribution of alkaline phosphatase in tissue, limited information can be obtained from a total ALP assay. Fortunately, the tissue sources of elevated ALP in serum can be determined by identifying the isoenzyme.
The isoenzymes of alkaline phosphatase are unique in that some organs have only one major isoenzyme rather than multiple isoenzyme forms. The isoenzymes of ALP differ in their physicochemical and electrophoretic titan gel upotreba bihar, and it is by taking advantage of these differences that individual isoenzymes can be identified. In addition to the liver, bone, intestinal and placental isoenzymes, macrohepatic, Regan, PA, Nagao, and renal isoenzymes have also been identified in serum.
The resolution, accuracy and convenience of the TITAN GEL Alkaline Phosphatase High Resolution procedure make it better than heat denaturation, isoelectric focusing and wheat germ lectin techniques for separating ALP isoenzymes. Kits include: 10 Gels, ALP Buffer, ALP Separation Enhancer, ALP Reagent, ALP Diluent, Blotter, Wicks, Templates. The TITAN-GEL high resolution agarose electrophoresis method for separation of alkaline phosphatase isoenzymes provides excellent resolution.
Neuraminidase is used to enhance separation of bone and liver fractions. A unique additive to the gel matrix ensures separation of the macrohepatic fraction from bone and liver. The excellent sensitivity of the system allows detection and separation of ALP isoenzymes even in samples with low activity. Up to eight samples may be kpotreba per gel.
It is prepared from pooled human serum and contains liver and bone isoenzyme bands. The control is a stabilized liquid. SDS Safety Data Sheets TITAN GEL Alkaline Phosphatase Isoenzyme. The alkaline phosphatase ALP titan gel upotreba bihar found in human serum originate from several sources with the greatest activity occurring in the bone, liver, intestine, and placenta.